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Veterinary Physiology and Biochemistry

About Department

The Department of Veterinary Physiology & Biochemistry started functioning in 1948 with the establishment of the Camp Veterinary College at Hisar affiliated to Punjab University, when the college was shifted from Lahore to Hisar, The department was shifted to the present building in the year 1958, and was named as department of Veterinary Physiology and Biochemistry headed by Dr. C.L. Vohra LVP-PVS-I. Dr. Kalicharan and Dr. D.P. Sharma were Assistant Professors and Dr. (Mrs.) S. Bhardwaj and Dr. Daljeet Singh Sahani were demonstrators. On establishment of Punjab Agricultural University (PAU) in 1962 with Hisar as Campus, the department was reorganized and was renamed Department of Veterinary Physiology & Pharmacology with Dr. Ajit Singh as Head, Dr. B.S. Paul as Professor Pharmacology, Dr. B. Mishra, Associate Professor of Physiology, Dr. B.D. Garg, Assistant Professor of Pharmacology, Dr. P.D. Kapoor & Dr. O.P. Nangia, Assistant Professors of Veterinary Physiology, Dr. M.S. Setia as Assistant Professor of Physiological Chemistry and Dr. V.K. Agarwal & Dr. V.P. Dixit as Lecturers. In 1970 the Departments of Veterinary Physiology and Pharmacology were separated with Dr. D.P. Sharma as Head of Veterinary Physiology. In 1972, Dr. S.S. Sukhija joined as Assistant Professor in Physiological Chemistry. The faculty gained strength by joining of Dr J. P. Puri & Dr S.L.Garg. Dr. Sandeep Gera, Dr. M. Gupta joint teaching fraternity in Veterinary Physiology in 1986, Dr. M.K. Rose and Dr. Sonia Sindhu in 1995. This arrangement continued till 1999, when as per VCI guidelines the Department of Veterinary Physiology was bifurcated into Department of Veterinary Physiology, headed by Dr. S.K. Garg and Department of Veterinary Biochemistry headed by Dr. V.K. Agarwal. Dr. N. Sangwan (VBC) joined in 2000. Dr. Jyotsana (VPY), Dr. Anita Ganguly and Dr. Sandeep Kumar (VBC) joined the faculty in 2009. Faculty in Department of Animal Production Physiology consistently produced research and teaching of international status since 1966 to June, 2011, when it became part of Physiology and biochemistry. In June 2011, the three departments of Veterinary Physiology, Animal Production Physiology and Veterinary Biochemistry were merged into single entity christened as Department of Veterinary Physiology & Biochemistry as per VCI guidelines 2009 with Dr. Sandeep Gera as Head. This brought on board the strength of endocrinology, semen biology and environmental Physiology through Dr. R.K. Tuli, Dr. Pardeep Singh, Dr. R.K. Malik and Dr. Meenakshi Virmani.

This department has a distinction to be housed in the very first building of the campus, whose foundation stone was laid by Dr.Punjab Rao Deshmukh, Union Minister of Agriculture on 2nd October, 1955. The department had the privilege of shifting in the newly constructed building in 1957 which accommodated the whole faculty of Veterinary and Animal Sciences at that time. Presently, this building is being shared by the department of Veterinary Anatomy and Veterinary Physiology & Biochemistry. The additional infrastructural facility are in terms of Nuclear Research Laboratory started by Dr P. K. Dwark Nath, once approved by AERB Mumbai for disposal of radioisotope waste and radiation in-vitro studies along with a animal production facility as Artificial Insemination pavilion, diagnostic laboratory, semen biology laboratory and Radioisotope laboratory in Animal Sciences premises of the LUVAS. These laboratories are well equipped with latest instruments and audio-visual aids. The department offers specialization in Veterinary Physiology and Veterinary Biochemistry with diagnostics, clinical biochemistry, environmental physiology, semen biology, infertility profiling, endocrinology, neem biology, ketosis experimental physiology, draft capacity, mastitis biochemical studies, reproductive biology, equine/bubaline stem cell research, digestive physiology. Parasitic control and bubaline proteomic profile of pregnancy specific protein, genetic polymorphism studies on hsp 70, LHR, LEPR genes, donor cell profiling for embryo cloning and characterization thereof as thrust areas for PG research.

 

 Head of the Department :

 
Dr. Manoj Rose
Phone (O): 01662-256121
E-Mail : hod.vpb@luvas.edu.in
 

 

 

 

 

 

 

 

 

 

 

Ø  VISION OF THE DEPARTMENT

  1. Metabolic and endocrine studies in ruminants to augment production efficiency.
  2. Enhancement of reproductive efficiency in farm animals through physiological interventions.
  3. Establishment of biochemical profile of livestock in health and disease.
  4. Exploration of biomarkers for development of affordable diagnostics and therapeutics.

 

Ø  Thrust areas for research

Ø  Endocrinology

Ø  Digestive Physiology and Endocrinology

Ø  Clinical Biochemistry

Ø  Structural and functional Proteomics

Ø  Nutritional interaction and clinical diseases

Ø  Climatology and Metagenomics

 

Faculty

Teaching

S. No.

Staff

Designation

Field of Specialization

1.

Dr. Nirmal Sangwan

BVSc & AH, MVSc, PhD, PDF

Professor

Vety. Biochemistry

2.

Dr. Meenkshi Gupta

BVSc & AH, MVSc, PhD

Professor

Vety. Physiology

3.

Dr. Pradeep Singh

BVSc & AH, MVSc, PhD

Professor

Vety. Physiology

4.

Dr. Sandeep Gera

BVSc & AH, MVSc, PhD, PDF

Professor

Vety. Biochemistry

5.

Dr. M K Rose

BVSc & AH, MVSc, PhD

Professor

Vety. Physiology

6.

Dr. Sonia Sindhu

BVSc & AH, MVSc, PhD

Professor

Vety. Physiology

7.

Dr. R K Malik

BVSc & AH, MVSc, PhD

Professor

Vety. Physiology

8.

Dr. Shalini Sharma

BVSc & AH, MVSc, PhD, PDF

Assistant Professor

Vety. Biochemistry

9.

Dr. Vijender Singh

BVSc & AH, MVSc, PhD, PDF

Assistant Professor

Vety. Biochemistry

 

Research

10

Dr. Jyotsana

BVSc & AH, MVSc, PhD

Scientist

Vety. Physiology

11

Dr. Sandeep Kumar

BVSc & AH, MVSc, PhD

Scientist

Vety. Biochemistry

12.

Dr. Meenakshi Virmani

BVSc & AH, MVSc, PhD

Scientist

Vety. Physiology

Supporting Staff

S. No.

Staff

Designation

1.

Sh. Surender Singh

Steno-typist

2.

Sh.S.P. Sangwan

Lab. Tech.

3

Sh. Rajender Kesar

Lab. Assistant

4

Sh. Hari Singh

Lab. Assistant

5

Sh. Krishan Lal

Lab. Assistant

6

Sh. Rattan Lal

Lab. Assistant

7

Sh. Satish Kumar

Lab. Assistant

8

Smt. Sushila kumari

Lab. Attendant

9

Sh. Sant Lal

Lab. Attendant

10

Sh. Suresh Kumar

V.L.D.A.

11

Sh. Suresh Singh

Animal Attendant

12

Sh. Kartar Singh

Animal Attendant

13

Sh. Balbir Singh

Animal Attendant

14

Sh. Ravi

Animal Attendant

15

Sh. Uday Singh

Animal Attendant

16

Smt. Santosh

Animal Attendant

Post Graduate Students

S. No.

Name of the student

1.

Ms Niharika Mohanty

2.

Rajesh Kumar

3.

Ashok Kumar

4.

Ms Fozia Shah

5.

Sandeep Kumar

6.

Nirmal Singh

7.

Ms Preeti Singh

8.

Rabinder Kumar

9.

Mayukh Gosh

10.

Nirmal Singh

11.

Vikash

12.

Sukhbir Singh Panghal

13

Ramkaran

14

Surbhi

15

Deeksha

16

Muhammad Abubakar Wakil

 

Number of post graduate students passed out since 2011

Degree

Number

MVSc

9

PhD

4

Major Research Achievements

Salient research findings/highlights

·     A total of 65 Murrah buffaloes tested for A1/A2 genotype of beta casein gene, all were found to be having A2A2 genotype.

·     Developed a simple and rapid technique for purification of anionic proteins from a complex mixture of proteins. The technique so developed could be utilized to segregate cationic and anionic proteins.

·     Using above-mentioned technique, we succeeded in purification of the beta-casein from whole milk of buffaloes and cattle nearly up to 90%.

·     A significant decrease in glucose and cholesterol concentrations was observed in both male and female kids after three months of age till 12 months.

·     Triglyceride and protein concentration didn’t change due to advancement of age in both male and female goats.

·     Estradiol concentration in both male and female kids decreased during first three months of age and then increased at six months of age.

·     Thyroxine hormone concentration in male gradually increased with age reaching peak at six months of age while in female it decreased at one month of age and thereafter gradually increased upto six months of age.

·     A gradual decrease in lymphocyte percent in goat kids with increasing age was observed till 12 months of age.

·     Progesterone concentration was higher at -30 day prepartum and lowest at zero day (kidding day).

·     Triglyceride and urea concentration was higher in twin producing goats during last month of pregnancy.

·     When sperms were incubated at 37 C, it resulted in significant decrease in progressive sperm motility, percent live spermatozoa, percent spermatozoa with intact acrosome and superoxide dismutase. While there was significant increase in the level of lipid peroxidation in sperms at 3 hours of incubation at 37ºC.

·     Sperm motility, percent sperm liveability and fructose concentration were found to be positively correlated with the zinc level in the seminal plasma, whereas fructose level was found to be negatively correlated.

·     Administration of dinoprost tromethamine (prostaglandin F2α) intramuscularly at the time of artificial insemination increased the conception rate in buffaloes.

·     Supplementation of dinoprost tromethamine (prostaglandin F2α) intramuscularly at the time of artificial insemination was observed to be more effective in primiparous animals and heifers as compared to pluriparous animals.

·     Triu B intravaginal implant and Crestar ear implant both were found to be effective in inducing estrus response in anestrus buffalo cows. However, Triu B intravaginal implant was more effective than Crestar ear implant for the treatment of anestrus animals in terms of conception rate.

Salient Research findings from Student’s research:

·     Supplementation of antioxidants in form of Vitamin E @ 3 mM and glutathione @ 1mM in tris extender helps in liquid preservation of buck semen upto 72hours at 4°C in refrigerator with higher progressive sperm motility, higher liveability percentage, higher percentage of sperms with intact acrosome.

·     Supplementation of antioxidants leads to decreased lipid peroxidation and increase in antioxidant enzymes, thus offering protection to the spermatozoa from the free radicals generated during storage of semen.

·     There was no significant difference of NIANP bull specific mineral mixture before and after feeding on semen quality.

·     Cysteamine would not be a suitable antioxidant as a supplement in extender for freezing bull semen as cysteamine has shown detrimental effects on semen quality and caused oxidative stress to the frozen sperms.

·     OptiprepTM (2.5% of 60% iodixanol) supplementation to freezing extender increases post-thaw sperm motility, membrane integrity, travelled more distance in cervical mucus in vitro condition and maintain better sperm motility in incubation test. Further, OptiprepTM supplementation minimizes oxidative stress during cryopreservation. 

·     Supplementation of 0.2–1% sericin also in semen extender improved frozen-thawed semen quality by preventing oxidative stress.

·     Total protein bands in Hyalomma anatolicum and Rhipicephalus (boophilus) microplus salivary gland crude extract were found to be 23 and 21 and there were variations in protein bands in both the species.

·     Hyalomma anatolicum salivary gland protein fraction nos. 28, 29, 30, 31, 32, 42, 58, 111 and 112 were found to have anti-platelet aggregation activities whereas in Rhipicephalus (boophilus) microplus salivary gland protein fraction nos. 22, 23, 25, 27, 31, 36, 38, 39 and 51 had the anti-platelet aggregation activities.

·     In Hyalomma anatolicum fraction nos. 28, 29, 30, 31, 32, 42, 58, 111 and 112 showed inhibitory effects on the release of intracellular calcium as evidenced by decrease in fluorescence emitted whereas in Rhipicephalus (boophilus) microplus intracellular calcium release inhibitory activities were found in fraction no. 22, 25, 27, 31, 36, 38, 39 and 51.

·     Platelet adhesion inhibition activities were observed in fraction nos. 28, 29, 30, 31, 32, 42, 58, 111 and 112 of Hyalomma anatolicum which were significantly lower than the antagonist. Similarly in Rhipicephalus (boophilus) microplus fraction nos. 22, 25, 27, 31, 36 and 51 inhibited the platelet adhesion whichwere significantly lower than the antagonist induced platelet adhesion inhibition. Fraction no. 38 and 39 of Rhipicephalus (boophilus) microplus showed inhibitory platelet adhesion activities similar to that of antagonist.

·     Mass spectrometric analysis of the proteins/peptides having the antiplatelet aggregating activities were found to resemble serum albumin precursor and globin subunit in Hyalomma anatolicum and Rhipicephalus (boophilus) microplus, respectively. These host protein homologues-proteins produced by the ticks seems to mimic host proteins.

·     Scanning electron photomicrograph showed platelet aggregation inhibition with Hyalomma anatolicum and Rhipicephalus (boophilus) microplus specific salivary gland protein fractions with rounding off of platelets having sponge like appearance without any pseudopodia.

·     In Hardhenu cattle population genotype frequencies were 0.30 (KK) and 0.70 (AK). The frequency of K and A allele were 0.65 & 0.35. A total of 15 KK and 35 AK animals were identified in Hardhenu cattle whereas no animal of AA genotype were observed. The overall DGAT1K allele frequency in crossbred cattle was 0.65.

·     Lysine variant was the likely ancestral form of DGAT1 gene and the presence of lysine allele in buffaloes and Indian zebu cattle breeds might be one of the factors for the high fat content of milk. Because of very high frequency to almost fixed nature of K allele in Sahiwal and other well defined Indian cattle breeds, DGAT1 K232A polymorphism may not be a suitable candidate for selection purpose.

·      However, considering the increasing trends of crossbred cattle population in India, elimination of the K allele or selection against it may be suggested to increase milk and protein yield in crossbred like Frieswal once association of K232A polymorphism with production traits is established. Moreover, consideration of selection pressure will be very important to counterbalance the negative effect on fat yield.

·     The hematological, endocrine, enzymological, metabolic, electrolyte and mineral profile for 31 nascent parameters over 48 samples of Hardhenu are documented for use as base data. The same can be used for physiological, paraclinical and clinical back up for this new crossbred strain of cattle.

·     The statistically significant breed wise higher value of hemoglobin, glucose, albumin in Sahiwal; TLC, creatinine, potassium, triglycerides in Hariana and ALT, bilirubin, thyroxin, progesterone in Hardhenu are observed. Age wise difference in these groups is presented for evaluation of gerontological status.

 

Research Projects

Ongoing projects

Ø  Role of motility stimulators in improving quality of frozen semen of Murrah buffalo bulls sponsored by Government of Haryana.

Ø  Physiological Investigation for augmenting reproduction & production in farm Livestock & poultry sponsored by Government of Haryana.

Ø  Biochemical changes in malnutrition and parasitic diseases sponsored by Government of Haryana.

Ø  Setting up of referral biochemical laboratory-strengthening of existing nuclear research laboratory for diagnostic aids to the farmers sponsored by Government of Haryana under RKVY.

Ø  Identification and characterization of novel peptides of clinical importance from salivary glands of Hyalomma ticks sponsored by University Grants Comission

Ø  Studies on Digestive Physiology of Ruminants sponsored by Government of Haryana.

Ø  Supply of injection material to farmers for induction of lactation in infertile cattle sponsored by Government of Haryana under self financed scheme.

Completed projects

Funding

Title of the project

Duration

Name of the PI

ICAR

Haematological studies on Hariana Cattle and Beetle Goats

1965-70

Dr Ajit Singh

State

Thyroid function of domestic animals and Poultry

1968-70

Dr Ajit Singh

PL-480

Early Development of Rumen Function In buffalo calves

1/1977 to 3/1981

Dr. O.P. Nangia

State

Draught capacity in castrated buffalo males, camels and cross bred calves

1974-75 to 1997

Dr. S.P. Agarwal

State

Hormonal Profile of livestock in relation to certain reproductive and systematic diseases.

1979-80 Non Plan Vety. Biochemistry after bifurcation

Dr. S.L. Garg

ICAR

Manipulation of rumen microbial ecosystem in order to increase efficiency of feed utilization.

1991 to 1995

Dr. O.P. Nangia

ICAR

Regulation of feed intake in buffaloes

1978-1981

Dr. O.P. Nangia

ICAR

Studies on effect of neem extract on physiological and Immunological parameters

2000-2003

Dr. Sandeep Gera

RKVY

4017-C(g)-VPB-1-OA- Upgrading of Biochemistry laboratory for providing diagnostic aids to the farmer

2014-2016

 

Dr. S. Kumar

UGC

7002-C(g)-VBC-5-OA- Identification and characterization of novel peptides of clinical importance from salivary glands of Hyalomma ticks’

2011-14

Dr. Nirmal Sangwan

 

External agencies funded

1.

4037-C (g)-VPB-2-OA- Setting up of facility for testing of animals for A1/A2 genotype of beta casein and Up-gradation of existing biochemistry laboratory for diagnostic facilities to the farmers

RKVY

PI: Dr. Nirmal Snagwan

2015

March 2018

2.

4040-C(g)AGB-2-OA- Genetic Improvement and Conservation of Indigenous Breed- Hariana

(In collaboration with Deptt of AGB)

RKVY

Associate PI: Dr. Meenaxi Virmani

2015

March 2018

4.

5515-C(b)-VPB-1-ICAR- Studies on rumen microbial  metagenomics in in relation to feed efficiency.

ICAR

PI:

Jyotsana Madan

2015

March 2017

         
         
         

State Funded Schemes

Ø  Role of motility stimulators in improving quality of frozen semen of Murrah buffalo bulls sponsored by Government of Haryana.

Ø  Physiological Investigation for augmenting reproduction & production in farm Livestock & poultry sponsored by Government of Haryana.

Ø  Biochemical changes in malnutrition and parasitic diseases sponsored by Government of Haryana.

Ø  Setting up of referral biochemical laboratory-strengthening of existing nuclear research laboratory for diagnostic aids to the farmers sponsored by Government of Haryana under RKVY.

Ø  Identification and characterization of novel peptides of clinical importance from salivary glands of Hyalomma ticks sponsored by University Grants Comission

Ø  Studies on Digestive Physiology of Ruminants sponsored by Government of Haryana.

Ø  Supply of injection material to farmers for induction of lactation in infertile cattle sponsored by Government of Haryana under self financed scheme.

Awards, Honours, Fellows and other recognitions

S. No

Name of Award

Faculty Members

Year of Award

Awarding Agency

1.

i)Rafi Ahmed Kidwai

Memorial Award

Dr.S.P. Agarwal

Dr.V.K.Agarwal

1996

 

2.

ii)Sh.Ram Lal Agarwal Memorial

Research Award of Indian Herbs

Research & Supply Co.

Dr.N.Singh/Dr.J.P.Puri/

Dr.S.L.Garg/Dr.O.P.Nangia

1979

 

 

iii)Sr.I.C.A.R. Fellowship

For Ph.D.

Dr.J.P.Puri

1985 to 1988

 

 

iv) Sr.CSIR Fellowship

For Ph.D.

Dr.Meenakshi Gupta

1993 to 1996

 

 

International Travel Fellowships

Name of faculty

Type of Assignment 

Period

Country

 

Dr.A.R.Rao

Deputation for teaching

1975-1977

Kabul – Afghanistan

Dr.O.P.Nangia

Training programme in Cardiovascular Physiology

3 months(1970)

U.S.A.

Dr.V.P.Dixit

Advanced diploma in Physiological Biochemistry (FAO scholarship)

1 year(1971-72)

Denmark

Dr.O.P.Nangia

Teaching Assignment

1981-83

Nigeria

Dr.V.P.Dixit

Ph.D.

1976 to 1979

Romania

Dr.J.P.Puri

Training on teaching methodology

3 months (1999)

U.S.A.

Dr.M.K.Rose

Ph.D.(DAAD fellowship)

1 year 3 months

(2003-04)

Germany

Dr.J.P.Puri

As Mentor under DAAD

Fellowship

6 days (2004)

Germany

Dr.J.P.Puri

To attend Conference

4 days

Lahore (Pakistan)

Dr. Sandeep Gera

Post Doctoral Fellowship

3 Months

Canada

Dr. Nirmal Sangwan

Post Doctoral Fellowship

9 Months

Edinburgh, UK

Dr. Sonia Sindhu

University of Sthrathclyde Award fellowship for PhD

2001

U.K.

Dr. Shalini Sharma

Award of EFIS (European federation of Immunological Societies) fellowship to attend 5th international conference on CMV and immunosenescence,

Nov 20-21st, 2014

Amsterdam, the Netherlands

 

ELEVATION OF FACULTY TO HIGHER OFFICE

Sr. No.

Name

Position

Institution

1.

Dr.O.P.Nangia

Held the post of Dean, COVS

 

CCSHAU

2.

Dr.P.K.Dwarkanath                       

post of ADR,

 

CCSHAU

3.

Dr.J.S.Bhatia 

selected as ADG

ICAR, New Delhi.

4.

Dr. Pradeep Bamal

Registrar

LUVAS

 

Books

Sr. No.

Title

Editors/Authors

Year of Publication

Publishers

1.

Practical Veterinary Physiology, 1st Edn. Pp. 1-210.

Rose, M.K., Gupta, M. and Sindhu, S.

2015

Kalyani Publishers, Ludhiana, New Delhi.

 

 

TEACHING Manuals

Sr. No.

Title

Editors/ Authors

Year of Publication

Publishers

1.

Practical Manual for ‘General Veterinary Biochemistry

Agarwal, V.K., Gera, S., Sangwan, N. and Garg, S.L

2002

-

2.

Practical Manual for ‘Veterinary Cinical Biochemistry

Sangwan, N., Gera, S. and Garg, S.L

2003

-

3.

Practical Manual for ‘Physiological Chemistry( Animal metabolism, systemic functions and enzymes)

Sangwan, N., Gera, S. and Garg, S.L.

2003

-

4.

Practical Manual for ‘Introduction to Molecular Biology and Biotechnology

 Gera, S., Garg, S.L. and Sangwan, N

2004

-

5.

Revised Practical Manual for ‘General Veterinary Biochemistry

Sangwan, N., Gera, S. and Kumar, S

2012

 

6.

Practical Manual for ‘Veterinary Intermediary Metabolism

Sangwan, N., Gera, S. and Kumar, S

2012

 

7.

Laboratory Manual of Veterinary Physiology Paper-I. (Unit-1 and Unit-2).

As per VCI Regulations -2016

 

Rose M.K., Sindhu, S. and Gupta, M.

2016

Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences, LUVAS, Hisar, India.

8.

Laboratory Manual of Veterinary Physiology Paper-II (Unit-3 and Unit-4).

 As per VCI Regulations -2016

Rose M.K., Sindhu, S., Gupta, M. and Malik, R.K.

2016

Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences, LUVAS, Hisar, India.

 

 

P.G. Thesis