Clinical and diagnostic

  • The total number of OPD and IPD cases treated during the year 2021-22 was 15869.
  • Total 2463 surgery cases were performed to alleviate various ailments in small and large animals.
  • Tissue adhesive for sealing of teat fistula in combination with absorbable sutures (polygalactin) in inverted pattern suturing on muscular layers was found effective.
  • Total of 983 gynecological and obstetrical cases were treated during the year 2021-22.
  • A total of 12423 medicinal cases were attended during the year 2021-22.
  • Total 11894 samples (7821 blood, 1151 faecal, 148 urine, 1743 milk, and 1031 serum) from OPD were processed in diagnostic laboratory, Karnal during the year 2021-22. 


  • A nested PCR RFLP assay has been standardization and validated for detection and differentiation of various Theileria spp. infecting small ruminants targeting 18S ribosomal RNA gene sequence.
  • Developed and standardized a Direct blood PCR assay for diagnosis of Theileria annulate and Trypanosoma evansi. This is highly sensitive, specific and rapid diagnostic tool as there is no need of DNA isolation from the whole blood.
  • Developed a Duplex PCR assay for simultaneous detection and differentiation of Babesia and Theileria infecting bovines.
  • Developed a Multiplex PCR assay for simultaneous detection of haemoprotozoan infections viz., annulata, T. evansi, Babesia bigemina and Anaplasma marginalein bovine
  • A PCR assay has been standardization and validated for detection of Theileria infecting small ruminants targeting 18S ribosomal RNA gene sequence
  • A PCR-RFLP technique has been developed for differentiation between various Theileria
  • First report of isolation and phylogenetic characterization of equine Setaria digitata in India based on mitochondrial COX1, 12S rDNA and ITS2 sequence data.
  • First report of molecular and phylogenetic analysis of Physaloptera praeputialis in naturally infected stray cats from India based on mitochondrial, ribosomal genes and nematode barcoding.
  • The blood direct PCR method for diagnosis of Anaplasma marginale in buffalo in conjunction with microscopic examination has been standardized and it may be useful for rapid and reliable detection.
  • Developed, standardized and optimized a universal oligonucleotide-based 18S rRNA PCR assay as a rapid diagnostic tool for the initial screening of piroplasmids infecting domestic and wild animals and is potentially helpful for large-scale epidemiological studies.
  • Developed a simple read out loop mediated isothermal amplification (LAMP) assay targeting a partial RoTat1.2 VSG gene of T. evansi with naked eye visualisation of LAMP products by adding SYBR Green I dye and can be useful for detecting exceedingly low parasitaemia in carrier animals.
  • Inhalation of isoflurane in combination of N2O through closed and semi closed methods in major surgical intervention in large animals found safe and effective in term of maintenance of pregnancy in buffaloes, better analgesia, sedation and muscle relaxation in all animals.
  • Use of tissue adhesive along with inverted suture pattern on mucosa and muscle layer using vickryl suture material, found effective in prevention of leakage and better healing in clinical cases of teat fistula.